Transcription of Type of an- Inactivator Concentration Comment …
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Type of an- Inactivator Concentration Comment …
EUROPEAN PHARMACOPOEIA Bacterial endotoxinsMaize gSodium gAgar, according to gelling g to gPurified water1000 mlHydrate the agar, dissolve by heating to boiling withcontinuous stirring. If necessary, adjust the pH so thatafter sterilisation it is Sterilise by heating in anautoclave at 121 C for 15 min. Allow to cool to 45-50 C;add, where necessary, gentamicin sulphate corresponding to20 mg of gentamicin base and pour into Petri R (Lactose monohydrate sulphite medium)Pancreatic digest of gYeast gSodium gLactose gCysteine gPurified water1000 mlDissolve, adjust to pH and fill to 8 ml in16 mm 160 mm tubes containing a small Durham by heating in an autoclave at 121 C for 15 min andstore at 4 use, heat the medium for 5 min in a water -bath andcool. Add to each tube ml of a 12 g/l solution ofsodiummetabisulphite Rand ml of a 10 g/l solution of ferricammonium citrate, both solutions being freshly preparedandfilteredthroughmembranes( m).
EUROPEAN PHARMACOPOEIA 5.0 2.6.14. Bacterial endotoxins Maize starch 1.0 g Sodium chloride 5.0 g Agar, according to gelling power 10.0 g to 15.0 g Purified water …
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