Transcription of Understanding secondary antibodies - …
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Understanding secondary antibodies - …
Understanding secondary antibodiesOur essential guide to immunoglobulin class, species reactivity, and conjugates3 Introduction to secondary antibodiesWhat are secondary antibodies ? secondary antibodies are raised to bind to primary antibodies to allow detection, sorting, and purification of target antigens. They allow the detection of your protein of interest due to their specificity for the primary antibody species and isotype. Different dyes or enzyme conjugates bound to the secondary antibody can then be chosen based on your needs for your specific determines secondary antibody performance? Specificity, which refers to the degree with which the antibody detects its target antigen and structurally dissimilar antigens. Sensitivity, which refers to the amount of antigen that the antibody is able to detect. Sensitivity not only depends on the binding affinity of the secondary antibody but also the label and degree of labeling of the antibody.
4. Choosing a secondary antibody. When you are choosing a secondary antibody for your experiment, here are some things . you should take into consideration.
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