Transcription of Understanding the Capillary GC Column
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Understanding the Capillary GC Column
Understanding the Capillary GC ColumnMark SInnottApplication EngineerThings to Is it Volatile enough to chromatograph by GC? Is it stable enough to survive vaporization? Is it a Gas or a Liquid? How are we getting the Sample Injected? What is the sample Matrix? Can we do sample clean up? Is it an established method?--EPA, ASTM, USP What do we Know about the analytes? What else MAY be present in the sample?Group/Presentation TitleAgilent RestrictedPage 2 Group/Presentation TitleAgilent RestrictedPage 3 Capillary Column TYPESL iquid PhaseCarrier GasPorous Layer Open Tube (PLOT)Wall Coated Open Tube (WCOT)Solid ParticlesCarrier GasGroup/Presentation TitleAgilent RestrictedPage 6 STATIONARY PHASE POLYMERSSiRROSiRROSiRRSiRROnmOSiRRnSilox aneSiarylene backboneR=methyl, phenyl, cyanopropyl, trifluoropropylOHCH2CH2O nPolyethylene GlycolGroup/Presentation TitleAgilent RestrictedPage 7 Stationary Phase% Substitution --polysiloxanes% = # of sites on silicon atoms occupiedBalance is methylOSiRRnSiloxaneR=methyl, phenyl, cyanopropyl, trifluoropropylGroup/Presentation TitleAgilent RestrictedPage 8 Stationary Phas
Poly(Ethylene) Glycol Modified •Base deactivated (CAM) •Acid Modified (DB-FFAP) •Extended Temperature Range. PLOT columns are primarily, but not exclusively, used for the analysis of gases and low boiling point solutes (i.e., boiling point of solute is at or below room temperature).
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