Transcription of Understanding the Capillary GC Column
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Understanding the Capillary GC Column
Understanding the Capillary GC ColumnMark SInnottApplication EngineerThings to Is it Volatile enough to chromatograph by GC? Is it stable enough to survive vaporization? Is it a Gas or a Liquid? How are we getting the Sample Injected? What is the sample Matrix? Can we do sample clean up? Is it an established method?--EPA, ASTM, USP What do we Know about the analytes? What else MAY be present in the sample?Group/Presentation TitleAgilent RestrictedPage 2 Group/Presentation TitleAgilent RestrictedPage 3 Capillary Column TYPESL iquid PhaseCarrier GasPorous Layer Open Tube (PLOT)Wall Coated Open Tube (WCOT)Solid ParticlesCarrier GasGroup/Presentation TitleAgilent RestrictedPage 6 STATIONARY PHASE POLYMERSSiRROSiRROSiRRSiRROnmOSiRRnSilox aneSiarylene backboneR=methyl, phenyl, cyanopropyl, trifluoropropylOHCH2CH2O nPolyethylene GlycolGroup/Presentation TitleAgilent RestrictedPage 7 Stationary Phase% Substitution --polysiloxanes% = # of sites on silicon atoms occupiedBalance is methylOSiRRnSiloxaneR=methyl, phenyl, cyanopropyl, trifluoropropylGroup/Presentation TitleAgilent RestrictedPage 8 Stationary PhasePoly(ethylene) Glycol100% PEG (DB-WAX)
Influence on α 1 2 k k R s N k k = 4 1 ... also contain atoms of nitrogen, oxygen, phosphorus, sulfur, or a halogen (Alcohols, amines, thiols, ketones, nitriles, organo-halides, etc. Includes dipole-dipole interactions and H-bonding) Polarizable Molecules - …
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