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Yeast Protocols Handbook

Yeast Protocols Handbook FOR RESEARCH USE ONLYPT3024-1 (PR742227)Published 11 February 2008 Yeast Protocols Handbook Clontech Laboratories, Inc. Protocol No. PT3024-1 2 Version No. PR742227 I. Introduction 4 II. Introduction to Yeast Promoters 5 III. culturing and Handling Yeast 10 IV. Preparation of Yeast Protein Extracts 12 A. General Information 12 B. Preparation of Yeast Cultures for Protein Extraction 12 C. Preparation of Protein Extracts: Urea/SDS Method 13 D. Preparation of Protein Extracts: TCA Method 15 E.

III. Culturing and Handling Yeast 10 IV. Preparation of Yeast Protein Extracts 12 A. General Information 12 B. Preparation of Yeast Cultures for Protein Extraction 12 C. Preparation of Protein Extracts: Urea/SDS Method 13 D. Preparation of Protein Extracts: TCA Method 15 E. Troubleshooting 17 V. Yeast Transformation Procedures 18

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