Bioprocessing Basics - Eppendorf
Many applications are well served by the cultivation of bacteria or yeast in shake flasks and cells in dishes or T-flasks. Bioreactors and fermenters, however, improve productivity and save work, time, and lab space for scientists, who: • need large quantities of cells, microbes, or the products they express • would like to improve the
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Cell Thawing Protocol Standardization – Guide for More ...
www.eppendorf.comthawing process in a reproducible procedure, which offers important benefits in situations where there is a higher de-mand for standardization and documentation of the thawing process. They are also well suited for situations where water baths are impractical [6,7]. The most important drawback to dedicated thawing devices is the upfront investment
A Beginner’s Guide to Bioprocess Modes – Batch, Fed- Batch ...
www.eppendorf.com[2]. This strain has an ampicillin resistance gene (bla) encoded on a plasmid. A concentration of 0.1 g/L ampicillin must be maintained in all cultures, since absence or low concentrations of ampicillin will result in plasmid loss [3]. Therefore, from inoculum preparation in shake flasks to fermentation runs in 3 L vessels, we always added 0.1 g/L
BioBLU c and BioBLU p Single-Use Vessels for cell culture
www.eppendorf.comLeachables and extractables are defined as substances that can either be extracted from plastic materials under harsh conditions (e.g. extreme temperatures, presence of solvent) or released spontaneously under standard conditions. They may originate from compounds used in polymer manufacturing such as softeners, stabilizers, and antioxidants. Espe-
Centrifuge 5804/5804 R Centrifuge 5810/5810 R - Eppendorf
www.eppendorf.com2.3.1 Declaration concerning the ATEX directive (2014/34/EU) Due to its design and the environmental conditions inside the device, the Centrifuge 5804/5804 R/5810/ 5810 R is not suitable for use in a potentially explosive atmosphere. The device may only be used in a safe environment, such as in the open environment of a ventilated
Bioreactors and Fermentors— Powerful Tools for Resolving ...
www.eppendorf.comPump Bioprocess control station Figure 1: Stirred-tank bioreactor system consisting of bioprocess control station, vessel, and bioprocess control software. The ... > Culture feeding, if required > Sampling, if required Culture harvest Figure 3: Series of events in a typical bioprocess run.
Scale-Up of Escherichia coli Fermentation from Small Scale ...
www.eppendorf.comPower number (Np) The (impeller) power number (also known as Newton number) is a dimensionless number associated with different type of impellers. Np is commonly used to calculate impeller power consumption during bioprocess scale-up [2]. We determined the power number and P/V values at various Used with 1 L fermentation glass vessel Used with 10 L
Cell Culture Scale-Up in Stirred-Tank Single-Use Bioreactors
www.eppendorf.comThe stirred-tank bioreactor design is relatively easy to describe with classical engineering approaches (Figure 1). Parameters to describe this vessel geometry include impeller diameter, vessel diameter, liquid height, and ratios thereof. It is the bioreactor design for which most research on Figure 1: Stirred-tank bioreactor design d D i h ...
Eppendorf Tubes
www.eppendorf.comWARNING! Damage to health from toxic, radioactive or aggressive chemicals as well as infectious liquids and pathogenic germs. Observe the national regulations for handling these substances, the biological security level of your laboratory, the material safety data sheets and the manufacturer's application notes.
Centrifugation Operating Manual - Eppendorf
www.eppendorf.comRead this operating manual completely before using the device for the first time. Observe the instructions for use of the accessories where applicable. This operating manual is part of the product. Please keep it in a place that is easily accessible. Enclose this operating manual when transferring the device to third parties.
Centrifuge 5425 - Eppendorf
www.eppendorf.comOperating instructions Centrifuge 5425 English (EN) 1 Operating instructions 1.1 Using this manual Read this operating manual completely before using the device for the first time. Observe the instructions for use of the accessories where applicable. This operating manual is part of the product. Please keep it in a place that is easily accessible.
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