Classification of Microorganisms
polyacrylamide gel electrophoresis • Premise: Genes determine protein sequence •Procedure: – Lyse cells, extract protein and dissolve in detergent – Place sample into a polyacrylamide gel – Electrophoresis separates out protein by charge and size …
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www.agilent.comPolyacrylamide (PAM) Water + 0.05 M Na 2 SO 4 at pH 3 PL aquagel-OH 25 5991-5789EN Polyacrylamide (PAM) Water + 0.2 M NaNO 3 + 0.01 M NaH 2 PO 4 at pH 7 PL aquagel-OH 40 5991-5773EN Polyacrylic acid (PAA) Water + 0.2 M NaNO 3 + 0.01 M NaH 2 PO 4, adjusted to pH 7 PL aquagel-OH 25 5991-5783EN Poly-alpha-olefi n (PAO) TCB + 0.015 % BHT PLgel …
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www.webdepot.umontreal.caGel de polyacrylamide. Figure 2-afin d’augmenter la résolution et donc restreindre les protéines dans des bandes plus minces, le gel peut être modifié ...