DNA EXTRACTION METHODS
•Resuspend nuclei, add Sodium Dodecly Sulfate (SDS), Proteinase K. Lyse nuclear membrane and digest protein. •DNA released into solution is extracted with phenol-chloroform to remove proteinaceous material. •DNA is precipitated from the aqueous layer by the additional of ice cold 95% ethanol and salt •Precipitated DNA is washed with 70%
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www.ou.ac.lkii The Open University of Sri Lanka Faculty of Engineering Technology Student Guidebook 2017 / 2018 Telephone no. Fax Dean / Faculty of Engineering Technology 011-2881228 011-2822737
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