DNA Plasmid Isolation Using Alkaline Lysis Method
DNA Plasmid Isolation Using Alkaline Lysis Method Buffers and Solutions Alkaline lysis solution I: 50 mM glucose, 25 mM Tris-Cl (pH 8.0), 10 mM EDTA (pH 8.0), de-ion water Alkaline lysis solution II : 0.2 N NaOH, 1% (w/v) SDS, de-ion water Alkaline lysis solution III : 5 M potassium acetate, glacial acetic acid, de-ion water Ethanol 70% (v/v)
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