Plasmid Isolation
Found 7 free book(s)
DNA Plasmid Isolation Using Alkaline Lysis Method
2015.igem.orgDNA Plasmid Isolation Using Alkaline Lysis Method Buffers and Solutions Alkaline lysis solution I: 50 mM glucose, 25 mM Tris-Cl (pH 8.0), 10 mM EDTA (pH 8.0), de-ion water Alkaline lysis solution II : 0.2 N NaOH, 1% (w/v) SDS, de-ion water Alkaline lysis solution III : 5 M potassium acetate, glacial acetic acid, de-ion water Ethanol 70% (v/v)
CRISPR / Cas9 KO Plasmid PROTOCOL and HDR Plasmid ...
datasheets.scbt.comCRISPR/Cas9 KO Plasmid and HDR Plasmid, and for the removal of genetic material flanked by LoxP sites. NOTE: Follow Phase I plasmid transfection protocol for Cre Vector (sc-418923) transfection. PHASE 4: Cell Assay Complete phenotypic and/or genotypic analysis may require isolation of single cell colonies to confirm complete allelic knockouts.
FM MC4 1. - Cold Spring Harbor Laboratory Press
www.cshlpress.comIsolation and Quantification of DNA 1 INTRODUCTION DNA Isolation 2 Commercial Kits for Purification of DNA 3 Quantifying DNA 5 PROTOCOLS 1 Preparation of Plasmid DNA by Alkaline Lysis with SDS: Minipreps 11 2 Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreps 15 3 Isolating DNA from Gram-Negative Bacteria (e.g., E. coli)19
Proteinase K
www.interchim.fr2.Isolation of plasmid and genomic DNA Genomic or plasmid DNA can be isolated from liquid nitrogen frozen cells or cultured cells using Proteinase K. Incubate 50-100 mg of tissue or 1×108 cells in 1mL of buffer containing 0.5% SDS (w/v) with Proteinase K at a concentration of 1mg/mL, for 12-18 hours at 50°C.
AdEasy Adenoviral Vector System - Agilent
www.agilent.comPlaque Isolation ... plasmid DNA is digested with Pac I to expose its inverted terminal repeats (ITR), and then used to transfect AD–293 (or HEK293) cells where deleted viral assembly genes are complemented in vivo. Advantages of Recombinant Adenovirus for Gene Expression
Plasmid Isolation (Alkaline Lysis) - G-Biosciences
www.gbiosciences.comPlasmid Isolation (Alkaline Lysis) Teacher s Guidebook (Cat. # BE-310) ... A purified plasmid should give a single band on an agarose gel, however larger bands may be visualized due to super-coiling or nicking. A small bright band will also be seen which is RNA.
One Shot Stbl3 Chemically Competent E. coli Product ...
assets.thermofisher.comWe recommend including the pUC19 control plasmid DNA supplied with the kit (10 pg/μL in 5 mM Tris-HCl, 0.5 mM EDTA, pH 8) in your transformation experiment to verify the efficiency of the competent cells. Do not use these cells for electroporation. 1. Thaw, on ice, one vial of One Shot™ Stbl3™ chemically competent cells for each ...