Dojindo Cell Proliferation Protocol
Dehydrogenase-based assays reflect cell conditions with more sensitivity than the other assays because they depend on several elements including dehydrogenase, NAD(H), NADP(H), and mitochondrial activity. The major difference between CCK-8 and the MTT assay, other than MTT’s toxicity, is the enzymes involved. The CCK-8 assay involves most of
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Cell Counting Kit-8 Technical Manual
www.dojindo.eu.comChemicals : Mitomycin-C (MMC), Dodecylsulfate, sodium salt (SDS) Incubation : 37°C, 5% CO 2, 2 hours Detection : 450 nm, Reference : 650 nm Precautions 1. Since the CCK-8 assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals
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