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Cell Counting Kit-8 Technical Manual
Chemicals : Mitomycin-C (MMC), Dodecylsulfate, sodium salt (SDS) Incubation : 37°C, 5% CO 2, 2 hours Detection : 450 nm, Reference : 650 nm Precautions 1. Since the CCK-8 assay is based on the dehydrogenase activity detection in viable cells, conditions or chemicals
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Measuring Cell Viability / Cytotoxicity
www.dojindo.eu.comand can also be applied to High-Throughput Screening such as a 384-well microplate. WST-8 is not cell permeable, which results in low cytotoxicity. Therefore after the assay it is possible to continue further experiments using the same cells. Applications: cell counting, cell proliferation experiments, cytotoxicity tests, drug sensitivity tests
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