Gram Stain Protocols
the solution as iodine oxidizes (McClelland, 2001) and uses an aqueous solution of Safranin for the counterstain (Gephardt et al., 1981). The reagents listed below can be made or purchased commercially from biological supply houses 1. Primary Stain: Crystal Violet Staining Reagen t. Solution A for crystal violet staining reagent
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Catalase Test Protocol - American Society for Microbiology
asm.orgThe catalase test is also valuable in differentiating aerobic and obligate anaerobic bacteria, as anaerobes are generally known to lack the enzyme (8, 9). In this context, the catalase test is valuable in differentiating aerotolerant strains of Clostridium, which are catalase negative, fromBacillus, which are catalase positive (8). Theory
Luria Broth (LB) and Luria Agar (LA) Media and Their Uses ...
asm.orgDownloaded from www.asmscience.org by IP: 71.127.236.37 On: Mon, 12 Aug 2019 20:18:49 American Society for Microbiology © 2016 1 Luria Broth (LB) and Luria Agar (LA ...
Indole Test Protocol
asm.orgDissolve components and store solution at room temperature in a brown glass bottle. To test for indole production, inoculate a 4 -ml tryptone broth tube with one loopful of culture. After 24 to 48 hours of incubation, add to the culture 1.0 ml of either ether or xylene. Mix well and then allow organic solvent to rise to the top of the medium.
Blood Agar Plates and Hemolysis Protocols
asm.org"Blood Agar" is not a consistently defined medium. The term "blood agar" generally refers to an enriched base medium to which defibrinated mammalian blood has been added. In the US "blood agar" is usually prepared from Tryptic Soy Agar or Columbia Agar base with 5% Sheep blood. Rabbit or horse blood may be
Microorganisms, Mold, and Indoor Air Quality
asm.orgings with poor indoor air quality anywhere in this or other countries. 2 Water stained ceiling tile from an office building with chronic roof leakage. Culture of Trichoderma showing fluffy,spreading growth on the agar surface. Visible mold growing on a floor joistin the crawl space under a residence with chronic water seepage.
Kirby-Bauer Disk Diffusion Susceptibility Test Protocol
asm.orgInstitute Performance Standards for Antimicrobial Disk Susceptibility Tests: Approved Standards 9th Edition (3). RECIPE Sterile saline in 2-ml tubes 0.5 McFarland standard Wickerham card Mueller-Hinton agar plates, 100 mm or 150 mm Caliper or ruler Antibiotic disksb Forceps Antibiotic disk dispenser (optional) 18- to 24-ho Vortex
Urease Test Protocol
asm.orgPeptone 1 g Dextrose 1 g Sodium chloride 5 g Potassium phosphate, monobasic 2 g Urea 20 g Phenol red 0.012 g Agar 15 to 20 g To prepare the urea base, dissolve the first six ingredients in 100 ml of distilled water and filter sterilize (0.45-mm pore size). Suspend the agar
Oxidase Test Protocol - American Society for Microbiology
asm.orgFIG. 3. On the left is oxidase-positive Pseudomonas aeruginosa and on the right is oxidase-negative Escherichia coli. Both organisms were rubbed on a dry filter that was then treated with one drop of Kovács oxidase reagent. Direct Plate Method (7, 10) (Fig. 4) 1. Grow a fresh culture (18 to 24 hours) of bacteria on nutrient agar
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An iodine / thiosulfate titration
mccscience.yolasite.comThe iodine solution, which is a golden-brown colour, can be titrated against sodium thiosulfate solution. The sodium thiosulfate solution is placed in the burette and, as it is added to the conical flask, it reacts with the iodine and the colour of the solution fades. When it reaches a
IODINE CLOCK REACTION KINETICS
alpha.chem.umb.eduIodine Clock 6 o Thiosulfate molarity drops from [S2O3 2-]i to zero. o The I2 moles per liter consumed is [S2O3 2-]i /2 (see the balancing coefficients in Reaction 2). o The I2 consumed in Reaction 2 is produced in Reaction 1. o For each I2 mole produced, a mole of H2O2 is consumed (see balancing coefficients in Reaction 1). o Thus the H2O2 loss, Δ[H2O2], is [S2O3