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Streptococcus Laboratory General Methods
www.cdc.govThe blood agar plates used in the manner described above are for checking for purity of cultures. If any of the results are unusual or not expected or the culture is contaminated the test must be repeated. Vancomycin resistant streptococci or other unknowns other than leuconostocs and pediococci, which are intrinsically vancomycin resistant.
ANNEX Preparation of Media and Reagents
www.cdc.govA. Blood agar plate (BAP): trypticase soy agar (TSA) + 5% sheep blood . A BAP is used as a general blood agar medium. It is used for growth and testing of N. meningitidis and S. pneumoniae. The plate should appear a bright red color. If the plates appear dark red, they are either old or the blood was likely added when the agar was too hot. If ...
Blood Agar Plates and Hemolysis Protocols
asm.orggreen or brown discoloration in the medium. The color can be equated with "bruising" the cells. Microscopic inspection of alpha -hemolyzed red blood cells shows that the cell membrane is intact, so it is not, in fact, true lysis. Some text book authors refer to alpha as “partial hemolysis,” which may be confusing to the student.
SELECTIVE AND DIFFERENTIAL MEDIA
fire.biol.wwu.eduobserved on blood agar plates (nutrient agar supplemented with a 5% concentration of sheep blood). Alpha hemolysins partially lyse the red blood cells and reduce the hemoglobin to methemoglobin which produces a green zone around the colony. Beta hemolysins cause complete lysis of the red blood cells
THE IDENTIFICATION OF TWO UNKNOWN BACTERIA
openlab.citytech.cuny.edu• Blood Agar (BA) to determine the hemolytic properties of both unknown bacteria • MacConkey Agar (MAC) that will select for the gram-negative bacterium and also determine whether or not it is a lactose fermenter. Colonies produced on this plate will also be used for biochemical testing to determine its identification.
Microbial Growth: cell number Chapter 6: Microbial Growth
www.lamission.edu4 Blood Agar:Used to distinguish bacteria that destroy red blood cells (hemolysis). Hemolysis appears as an area of clearing around colony. Example: Streptococcus pyogenes. Microbial Growth Culture Media Both Selective and Differential Media : Used both to distinguish colonies of a desired organism, and inhibitthe growth of other microbes.