How to isolate proteins
with immobilized reactive dyes, covalently bound nucleotides, metals and numerous ... -binding proteins is the use of agarose or sepharose-bound reactive dyes. The protein sample is loaded onto a 10 mm x 20 cm column packed, for instance, with Cibacron Blue-agarose 3GA resin (immobilized on cross-linked 4% beaded agarose, type ...
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How to prepare aceto-orcein squashes, especially …
www.fgsc.netHow to prepare aceto-orcein squashes, especially for pachytene chromosomes. Edward G. Barry and David D. Perkins Background Aceto-orcein squashes of meiotic chromosomes provided the first definitive information on the karyotype
How to choose and prepare media - Fungal …
www.fgsc.netLast revised 4/18/06 How to choose and prepare media. David D. Perkins Background Two synthetic media are now in general use as standards for culturing and crossing Neurospora -- Medium N (Vogel
How to cross-link proteins - Fungal Genetics Stock …
www.fgsc.netfor determination of the quaternary structure and arrangement of subunits within homo-oligomeric proteins and intra-subunit crosslinks for maintenance of stable tertiary
How to isolate mRNA - Fungal Genetics Stock Center
www.fgsc.netMN 04/22/04 How to isolate mRNA Minou Nowrousian Background: mRNAs (messenger RNAs) comprise only a small percentage of all RNA species in a eukaryotic cell, in Neurospora usually ~ 1-6 % (Lucas et al., 1977; Sturani et al., 1979).For some applications like preparation of a cDNA library, target preparation
How to preserve stocks. David Perkins Background
www.fgsc.netPost. McC, DDP revision,10 Oct 05 1 How to preserve stocks. David Perkins Background Storage of stock cultures in suspenced animation is essential, both for wild type references and for mutant strains.
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