IMMUNOPRECIPITATION (IP) PROTOCOL
Sepharose beads, if using a polyclonal antibody protein A-coupled Sepharose beads are usually suitable (please refer to ‘Choosing the protein beads’ table. If the beads come as a powder incubate 100 mg of beads in 1 ml PBS 0.1M, wash for one hour so they swell up, then centrifuge, remove the supernatant and discard. Add 1ml PBS-BSA
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