Search results with tag "Sepharose"
Gelatin Sepharose - Pennsylvania State University
personal.psu.eduGelatin Sepharose Gelatin Sepharose™ 4B is gelatin coupled to Sepharose 4B by the cyanogen bromide method. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma. Gelatin Sepharose 4B has been designed for the ...
HiTrap HIC columns - S. Mansy
www.smansy.org11-0034-52 AF 7 Butyl-S Sepharose 6 Fast Flow is based on a 90 µm matrix. The main differences between Butyl-S Sepharose 6 Fast Flow and Butyl Sepharose 4 Fast Flow lie in the length of their spacer arms, the
IMMUNOPRECIPITATION (IP) PROTOCOL
www.abcam.comSepharose beads, if using a polyclonal antibody protein A-coupled Sepharose beads are usually suitable (please refer to ‘Choosing the protein beads’ table. If the beads come as a powder incubate 100 mg of beads in 1 ml PBS 0.1M, wash for one hour so they swell up, then centrifuge, remove the supernatant and discard. Add 1ml PBS-BSA
![[PDF] Q Sepharose High Performance ... - gelifesciences.co.jp](/cache/preview/2/7/a/d/c/6/f/e/thumb-27adc6fe19db582e77a08f6918d91da8.jpg)
[PDF] Q Sepharose High Performance ... - gelifesciences.co.jp
www.gelifesciences.co.jp4 Data File 18-1172-88 AB A 254 nm 0.2 0.1 0 50 100 150 200 250 300 350 400 Volume (ml) CCC OC Column: HiLoad 16/10 Q Sepharose High Performance
Application Note 組織・細胞からのタンパク質抽出方法
www.atto.co.jpしたタンパク質をNi-Sepharoseカラムで 精製した結果を示しています。EzBactYeast Crusher は、カラムへの結合、各精製ステッ プの反応を阻害することなく、また酵素 活性も保持した状態でタンパク質を精製 できます。このようにEzBactYeast Crusher
Affinity Chromatography: Principles and Applications
cdn.intechopen.comal, 1968). Other early articles described the activation of a Sepharose matrix using a cyanogen bromide (CNBr) reaction (Axen et al, 1967) and the use of a spacer arm to alleviate steric hindrance (Cuatr ecasas et al, 1968). Affinity chromatography is still developing. It has played a central role in many Omics
HisTrap HP, 1 ml and 5 ml - Auburn University
webhome.auburn.eduHisTrap™ HP is a ready to use column, prepacked with precharged Ni Sepharose™ High Performance. This prepacked column is ideal for preparative purification of Histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC). The special design of the column, together with the high-performance matrix of the
Use of sodium hydroxide for cleaning and sanitizing ...
www.proteinguru.comFurther work on Q Sepharose Fast Flow, a strong anion exchanger, has shown that 1 M sodium hydroxide combined with 1 M sodium chloride effectively removes DNA, but that the level of removal is dependent on the nature of the sample (6). …