IMMUNOPRECIPITATION (IP) PROTOCOL
20 mM Tris HCl pH 8 137 mM NaCl 10% glycerol 1% Nonidet P-40 (NP-40) 2 mM EDTA Store up to 6 months at 4 oC Immediately before use add: Protease inhibitors 2. RIPA (RadioImmunoPrecipitation Assay) buffer More denaturing than NP-40 or Triton X-100 lysis buffer, RIPA buffer contains the ionic detergents SDS and
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