Indole Test Protocol
Agar 2.0 g Bromcresol purple 0.02 g Bring to 1 liter with distilled water. Heat mixture to boiling to dissolve agar. Cool to 50°C. Dispense in 4.0 to 5.0 ml aliquots in 16-mm test tubes. Cap tubes and autoclave at 121°C under 15 psi pressure for 15 minutes. After autoclaving, allow tubes to cool in an upright position to form the agar deep.
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