Example: stock market
Ni-NTA Purification System

Ni-NTA Purification System

Back to document page

bursts at medium intensity, then flash freeze the lysate in liquid nitrogen or a methanol dry ice slurry. Quickly thaw the lysate at 37°C and perform two more rapid sonicate-freeze-thaw cycles. 4. (Optional) If the lysate is very viscous, add RNase A (10 μg/mL) and DNase I (5 μg/mL) and incubate on ice for 10–15 minutes. Alternatively,

  Flash, Ni nta

Download Ni-NTA Purification System


Information

Domain:

Source:

Link to this page:

Please notify us if you found a problem with this document:

Other abuse

Advertisement

Related search queries