Search results with tag "Ni nta"
Ni-NTA 亲和层析介质 - genscript.com.cn
www.genscript.com.cn金斯瑞ni-nta 亲和层析介质（产品编号l00250）是把nta（氮川三乙酸）共偶联到 4%的琼脂糖介质 上，再通过nta 的4 个结合位点螯合ni2+制备而成的亲和层析介质。ni-nta 亲和层析介质的ni2+脱落 …
Ni-NTA Purification System - Thermo Fisher Scientific
tools.thermofisher.com3. Using a sonicator equipped with a microtip, sonicate the solution on ice using six 10-second bursts at high intensity with a 10-second cooling period between each burst. Alternatively, sonicate the solution on ice using two or three 10-second bursts at medium intensity, then flash freeze the lysate in liquid nitrogen or a methanol dry ice ...
Ni-NTA Purification System
tools.thermofisher.combursts at medium intensity, then flash freeze the lysate in liquid nitrogen or a methanol dry ice slurry. Quickly thaw the lysate at 37°C and perform two more rapid sonicate-freeze-thaw cycles. 4. (Optional) If the lysate is very viscous, add RNase A (10 μg/mL) and DNase I (5 μg/mL) and incubate on ice for 10–15 minutes. Alternatively,