Protein extraction from Tissues and Cultured Cells using ...
» We recommend using 100 µl of an appropriate lysis buffer per 1x10^6 cells. » For Western blotting, cells might be lysed directly in 1x Laemmli buffer. After sonication, centrifuge extract at 14,000 rpm for 15 min. Transfer the supernatant to a new tube and boil for 3 min. The supernatant can be used in Western blot.
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RNA extraction from tissue - PROTOCOL using Bioruptor ...
www.diagenode.comPROTOCOL Europe - Diagenode s.a. / orders@diagenode.com / info@diagenode.com // North America - Diagenode Inc. / orders.na@diagenode.com / info.na@diagenode.com www.diagenode.com 1 RNA extraction from tissue - using Bioruptor® (Standard/Plus) and RNA extraction kit Introduction Isolation of intact RNA is essential for many techniques used in …
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