Proteinase K
to clean nucleic acid complexe samples and to lyse cells. It has been used for isolation of mRNA, high molecular weight DNA and to inactivate ... Purification of genomic DNA from bacteria (miniprep): bacteria from a saturated liquid culture are lysed and proteins are removed by a digest with 100 μg/ml Proteinase K for 1 h at 37 °C. The enzyme ...
Download Proteinase K
Information
Domain:
Source:
Link to this page:
Documents from same domain
DiI, DiD, DiR, DiO, DiA - interchim.fr
www.interchim.frFluoProbes® FT-46804A DiI, DiD, DiO, DiR, DiA Lipophilic carbocyanine fluorescent dyes for membrane labeling Product Information Product name cat.number
NT-47255g Protein Electrophoresis in Agarose Gels
www.interchim.frNT-47255g Protein Electrophoresis in Agarose Gels + Introduction Protein electrophoresis in agarose gels is an alternative approach to using polyacrylamide gels and provides several
FT-062000 Good's buffers (biological buffers)
www.interchim.frFT-062000 P.1 Good's buffers (biological buffers) Products Description / Overview Organic biological buffers replace mineral buffers advantageously in many applications.
FT-185582 Aprotinin
www.interchim.frFT-185582 p.2 Inhibitory constants: Enzyme activity Ki [M] Aprotinin is sometimes given in Trypsin Inhibitor Unit (TIU)[].One TIU will decrease the activity of two trypsin units
OPA, amine detection reagent - INTERCHIM: Home
www.interchim.frFT-02727A OPA, amine detection reagent Sensitive fluorescent detection reagent for amines (i.e. aa, proteins and peptides) Works also …
HPLC - Les technologies Interchim 33 (0)4 70 03 73 09
www.interchim.fremail. interchrom@interchim.com - www.interchim.com e-mail interchrom@interchim.com B.3 B.3 Analyse Colonnes HPLC HPLC - les technologies Interchim
DTT (DithioThreitol)
www.interchim.frFT-054721(284251) P.2 Reducing properties DTT is an unusually strong reducing agent, with a redox potential of -0.33 V at pH 7. The pKa of thiol groups is
HPLC préparative 33 (0)4 70 03 73 01
www.interchim.fremail. interfine@interchim.fr - www.interchim.com e-mail interfine@interchim.fr D.19 D.19 Purification Process HPLC préparative Les grandeurs fondamentales
FT-01566H Quantitation of sulfhydryls DTNB, Ellman’s reagent
www.interchim.frFT-01566H For any question, contact your local distributor at uptima@interchim.com Uptima, powered by Hotline : +33(0)4 70 03 76 06; http://www.interchim.com
GC Stationary Phase Selection guide - INTERCHIM: Home
www.interchim.frUptiBondTM is Interchim's proprietary GC capillary column technology that has been developed around rigorous, innovative manufacturing processes from the highest quality raw material. Every step of column manufacturing is strictly controlled, establishing a new standard in GC chromatography. ... GC Stationary Phase Selection guide
Related documents
Protocol for Nextera™ DNA Sample Prep Kit (Illumina ...
nextgen.mgh.harvard.eduIf DNA purity is in question, the DNA should be cleaned using Zymo Genomic DNA Clean & Concentrator, Cat. No. D4010 (or equivalent). 3. Transposon End Sequence: The 19-bp transposon DNA sequence is present at the 5' end of all Illumina-compatible libraries. However, the 19-bp transposon DNA sequence is NOT sequenced on the Illumina platform.
MLPA DNA protocol - MRC Holland
www.mrcholland.comTris-HCl, EDTA, synthetic/control plasmid DNA, human genomic female DNA, cell line DNA * Probemixes are designed for use only in combination with SALSA MLPA reagent kits. # A ... clean with water and follow appropriate site procedures. 2 Schouten JP et al. (2002). Relative quantification of 40 nucleic acid sequences by multiplex ligation ...
Nextera DNA Library Prep Reference Guide (15027987)
support.illumina.comTips and Techniques NexteraDNALibraryPrepReferenceGuide 7 TipsandTechniques Unlessasafestoppingpointisspecifiedintheprotocol,proceedimmediatelytothenext
Nextera XT DNA Library Prep Kit Reference Guide (15031942)
support.illumina.comAssess DNA Purity UV absorbanceis a common methodusedfor assessing the purity of a DNA sample.The ratio of absorbance at 260 nm to absorbanceat 280 nm provides an indication of sample purity.This protocol is optimizedfor DNA with 260/280 absorbanceratio values of 1.8–2.0,which indicates a pure DNA sample.
DNA quality: electrophoresis, spectrophotometry and ...
www.nbpgr.ernet.inIn case of plant genomic DNA, the nucleic acids are electrophoretically separated on a 0.7-0.8% agarose gel containing ethidium bromide at a final concentration of 0.5 g/ml. The quantity of DNA can be estimated by comparing the fluorescent yield of the samples with a series of standards, for instance, lambda ( ) DNA at varying known
Molecular Laboratory Design, QA/QC Considerations
www.aphl.orgMar 07, 2017 · Clean the PCR workstation at the start and end of each work day/run (UV light, 70% ethanol, fresh 10% sodium hypochlorite, ... contaminating PCR amplicons are degraded leaving only genomic DNA available for PCR: Quality Controls: Monitor all …