Relative quantification
constant level of fluorescence or C P acquisition according to the established mathematic algorithm (see Section 3.6). Three general procedures of calculation of the relative quantification ratio are established: 1. The so-called ‘delta C t’ (eqs. 1–2 using ∆C P) or ‘delta-delta C t’ method (eqs. 3–4 using ∆∆C
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