RIPA Buffer (10X) - Cell Signaling Technology
7. Centrifuge extract for 10 minutes at 14,000 x g in a cold microfuge. 8. Remove supernatant for use. Additional notes: 1. For non-adherent cells, add 400 µl of buffer per 107 cells once they have been washed in 1X PBS and pelleted. 2. 1X RIPA Buffer can be used for lysis of tissue samples, although a homogenization step is recom-
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