Sample Preparation Guidelines for Cell Sorting
Sep 18, 2017 · High pressure during sorting compromises buffer capacity. Add 10-25mM HEPES to improve pH stability. To samples with reduced cell viability, omit EDTA and add 25-50 ug/mL DNAseI with 5mM MgCl. 2. This digests free DNA released by dead cells. Single Cell Suspension .
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