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Basic Data Analysis, Gating, and Statistics in Flow Cytometry
cancer.wisc.eduFITC 0 200 400 600 800 # Cells Detector Path laser 1 Detector Path laser 2 Pinhole Photocathode Anode Dynodes Voltage in Signal out Time y From ExcyteExpert Cytometry 1. Emission 2. Detection 3. Converted to Voltage 4. Measured 5. File Generated 6. Plotted •Detection of light from scatter or fluorescence in cells is converted into a voltage ...