SDS-PAGE PROTOCOL Adapted from Current Protocols, Ch. …
5. Block blot by soaking in blotting buffer for 1 hr with shaking. Alternatively, blocking can be done with as much as 10% milk and 0.5% Tween 20 to reduce background. 6. To 10 ml blocking solution, add primary antibody at proper dilution. Incubate the membrane for 1 hr with shaking. Alternatively, incubation with 1º Ab can be done ON @ 4 ºC, 7.
Download SDS-PAGE PROTOCOL Adapted from Current Protocols, Ch. …
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