Trizol
Found 11 free book(s)
RNA Isolation with TRIzol (Invitrogen) and Qiagen RNAeasy
ocg.cancer.govTRIzol reagent is a mono-phasic solution of phenol and guanidine isothiocyanate. During tissue homogenization or lysis, the TRIzol reagent maintains RNA integrity, while disrupting cells and dissolving cell components. Addition of chloroform followed by centrifugation separates the solution into an aqueous phase and an organic phase. ...
Total RNA extraction using Trizol reagent
health.uconn.edu0.5 ml of isopropyl alcohol per 1 ml of TRIZOL Reagent used for the initial homogenization. Incubate samples at 15 to 30°C for 10 minutes and centrifuge at no more than 12,000xg for 10 minutes at 2 to 8°C. The RNA precipitate, often invisible before centrifugation, forms a gel-like pellet on the side and bottom of the tube. 4. RNA WASH.
Interpreting Nanodrop (Spectrophotometric) Results
www.u.arizona.eduphenol, TRIzol, and chaotropic salts, the peptide bonds in proteins absorb light between 200 and 230 nm. A260/280 ratio The A260/280 ratio is generally used to determine protein contamination of a nucleic acid sample. The aromatic proteins have a …
260/280 and 260/230 Ratios - dna.uga.edu
dna.uga.eduThe TRIzol reagent is a phenolic solution which ab-sorbs in the UV both at 230 nm and ~270 nm (Figure 3). Figure 2 - EDTA spectral pattern Fi Guanidine HCL used for DNA isolations will absorb at ~230 nm (Figure 4) while guanidine isothiocyanate, used for RNA isolations
2）培養細胞からの RNA の回収と cDNA の合成操作
www.fmric.or.jp2）TRIzol® Reagent 1mL に対して，0.2mL のクロロホルム を加える． 3）チューブのフタをしっかり閉め，15 秒間手で 激しく振って撹拌し，室温で2-3 分間静置する． 5）遠心分離後，無色透明の上層（水相）と 白色の中間相，赤色の下層（有機相）に
Safety Data Sheet - Thermo Fisher Scientific
tools.thermofisher.comApr 25, 2017 · Product name TRIZOL REAGENT Company/undertaking identification 24 hour Emergency Response: 866-536-0631 301-431-8585 Outside of the U.S. +1-301-431-8585 24 hour Emergency Response for Hazardous Materials [or Dangerous Goods] Incident. Spill, Leak, Fire, Exposure, or Accident. Call CHEMTREC Within the USA + Canada: 1-800-424-9300 and +1 …
Trizol使用说明书 - shinegene.org.cn
www.shinegene.org.cn再研磨，如此三次，按50-100mg组织/ml Trizol加入Trizol，转入离心管进行第2步操作。 2) 匀浆：组织样品按50-100mg/mlTrizol 加入Trizol。另外，组织体积不能超过Trizol体积 的10%，否则匀浆效果会不好，用电动匀浆器充分匀浆约需1-2分钟。 五、从细胞中提取总RNA
TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A
tools.thermofisher.commolecular size. TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. TRIzol™ Reagent allows for simultaneous processing of a large number of samples, and is an improvement to the single-step RNA isolation
TRIzol Reagent (DNA isolation) User Guide - Pub. no ...
assets.thermofisher.comwithin one hour. TRIzol™ Reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components which facilitate the isolation of a variety of RNA species of large or small molecular size. TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting
TRIzol Reagent User Guide - Pub. no. MAN0001271 - Rev. A
tools.thermofisher.commolecular size. TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. TRIzol™ Reagent allows for simultaneous processing of a large number of samples, and is an improvement to the single-step RNA isolation
TRIzol Reagent Product description - Thermo Fisher Scientific
assets.thermofisher.comTRIzol™ Reagent allows for simultaneous processing of a large number of samples, and is an improvement to the single-step RNA isolation method developed by Chomcynski and Sacchi (Chomczynski and