2)培養細胞からの RNA の回収と cDNA の合成操作
2)TRIzol® Reagent 1mL に対して,0.2mL のクロロホルム を加える. 3)チューブのフタをしっかり閉め,15 秒間手で 激しく振って撹拌し,室温で2-3 分間静置する. 5)遠心分離後,無色透明の上層(水相)と 白色の中間相,赤色の下層(有機相)に
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