SELECTIVE AND DIFFERENTIAL MEDIA
Coagulase acts within host tissues to convert fibrinogen to thrombin. It is theorized that the fibrin meshwork that is formed surrounds the bacterial cells or infected tissues, protecting the organism from nonspecific host defense mechanisms such as phagocytosis. A coagulase-positive strain of Staphylococcus will cause fibrin formation,
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SELECTIVE AND DIFFERENTIAL MEDIA - …
fire.biol.wwu.edu50 EOSIN METHYLENE BLUE AGAR (EMB agar) Eosin methylene blue agar is a differential medium used for the detection and isolation of Gram-negative intestinal pathogens. A combination of eosin and
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BACTERIAL EXAMINATION OF WATER - OpenWebMail
fire.biol.wwu.edu83 FIRST PERIOD Material: 1. Nine tubes of double-strength lactose broth 2. 10, 1.0 and 0.1 ml pipets 3. Water samples Procedure: (work in groups of four) Presumptive Test 1. Take a water sample (dilute as instructed in some cases) and inoculate three
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ISOLATION OF PURE CULTURES
fire.biol.wwu.eduExposure of sterile media to the environment will demonstrate the importance of aseptic technique. 1. Label two nutrient agar plates as "Exposure I" and "Exposure II." 2. Uncover the plate marked "Exposure I" and allow it to remain exposed in the lab for about 5 minutes. 3. Expose the plate marked "Exposure II" to a source of possible contaminants.
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Comparing the size of a virus, a bacterium, and a ...
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www.ndhealth.govMethicillin-resistant coagulase negative staphylococcus mixed with a methicillin-susceptible Staphylococcus aureus. It is advisable to perform a purity check of the inoculum suspension each time a susceptibility test is performed. This plate should be examined for the presence of a second organism. Isolate and retest if indicated. 2 ...