Transformation Protocol Using Heat Shock
4) Keep tubes on ice. 5) Add 50 ng of circular DNA into E.coli cells. Incubate on ice for 10 min. to thaw competent cells. 6) Put tube(s) with DNA and E.coli into water bath at 42οC for 45 seconds. 7) Put tubes back on ice for 2 minutes to reduce damage to the E.coli cells. 8) Add 1 ml of LB (with no antibiotic added).
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