Transformation Protocol Using Heat Shock
1) Take competent E.coli cells from –80oC freezer. a. Use DH5α cells in most cases. b. If want to cut at XbaI or other DAM- enzyme site, use SCS110 cells which are deficient in Dam and Dcm methylases. 2) Turn on water bath to 42οC. 3) Put competent cells in a 1.5 ml tube (Eppendorf or similar). For transforming a
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