Why purify proteins? - UAB
Solubility of Proteins • Solubility of a protein depends on ionic strength of the solution • Ionic strength is defined as I = (1/2) ∑ i C i Z i 2 • The sum is over all ionic species • Because the charge Zi is squared, divalent and trivalent ions contribute greatly to I. • Ci is the molar concentration of species i
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