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Yeast Protocols Handbook

Yeast Protocols Handbook

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B. Plasmid Isolation From Yeast 34 C. Transforming E. coli with Yeast Plasmids 36 VIII. Analysis of Yeast Plasmid Inserts by PCR 39 A. General Information 39 B. Tips for Successful PCR of Yeast Plasmid Templates 39 IX. Additional Useful Protocols 42 A. Yeast Colony Hybridization 42 B. Generating Yeast Plasmid Segregants 43 C. Yeast Mating 44

  Isolation

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