MightyAmp™ DNA Polymerase Ver - catalog.takara …

1 R076A MightyAmp DNA Polymerase v201805Da2 R076 AMightyAmp DNA Polymerase PCR PCR PCR MightyAmp DNA Polymerase MightyAmp DNA Polymerase PCR PCR PCR PCR GC AT 10 Additive for High Specificity PCR PCR 98 C PCR I 200 1 MightyAmp DNA Polymerase U/ l 200 l2 MightyAmp Buffer (Mg2 , dNTP plus)

2 2 1 ml 510 Additive for High Specificity 500 l 2 1 50 l 2 Mg2 4 mM 2 dNTP 600 M each 2 II 20 CIII PCR 2 MightyAmp Buffer l1 Primer 115 MPrimer 215 M MightyAmp DNA Polymerase U/50 l 10 Additive for High Specificity5 l1 2 up to 50 lTotal50 l 1 EDTA 10 l 3 5 l 1 mm mm2 mm3 2 mm 5 l 3 2 10 Additive for High Specificity 10 Additive for High Specificity 3 R076 AIV OLIGO Primer Analysis Software Molecular Biology Insights Tm 60 C Tm C [ A T 2 ] [ G C 4 ] 35 2 [ Total base ]V PCR 68 C 3 step PCR GC 2 step PCR 3 step PCR 98 C2 min.

3 1 98 C10 C15 40 cycles68 C 21 2 step PCR 98 C2 min. 1 98 C10 40 cycles68 C1 1 98 C 2 min. 2 3 step PCR 68 C VI MightyAmp DNA Polymerase PCR TAE Buffer TBE Buffer PCR DNA Loading Buffer Proteinase K 1 6 Loading Buffer 9156 Proteinase K 9034 10 1 v/v 2 1.

4 PCR 1 5 v/v 4 R076 AVII MightyAmp DNA Polymerase PCR 3 A 1 PCR T-Vector pMD20 3270 pMD19 (Simple) 3271 Mighty Cloning Reagent Set (Blunt End) 6027 VIII Tm IV. 2 step PCR3 step PCR 3 step PCR2 step PCR 3 step PCR 2 step 2 C 40 cycles Sample Sample Sample Additive for High Specificity Tm IV.

5 3 step PCR2 step PCR 25 30 cycles Sample Sample Additive for High Specificity 10 Additive for High Specificity 5 R076 AIX IX-1 PCR PCR PCR PCR E. coli genomic DNA 2 105 copy 16S rDNA 173 bp 10 Additive for High Specificity MightyAmp PCR 98 C2 min.

6 98 C10 C15 cycles68 C30 sec. Taq 1 : 0 g 2 : g 3 : g 4 : g 5 : g /25 l M : 100 bp DNA Ladder 3407A 10 Additive M 1 2 3 4 5 M 1 2 3 4 5 MightyAmp Ver. 3B M 1 2 3 4 5 M 1 2 3 4 5 M A M 1 2 3 4 5 10 Additive 6 R076 AIX-2 NaCl PCR 500 mM NaCl PCR PCR PCR E.

7 Coli genomic DNA 2 105 copy 16S rDNA 1,465 bp 10 Additive for High Specificity MightyAmp PCR 98 C2 min. 98 C10 C15 cycles68 C90 sec. 1 : NaCl 0 mM 2 : 50 mM 3 : 75 mM 4 : 100 mM 5 : 125 mM 6 : 150 mM 7 : 175 mM 8 : 200 mM M : DL 2,000 DNA Marker 3427A 10 Additive for High Specificity 10 Additive for High Specificity M 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 MM 1 2 3 4 5 6 7 8 Taq 7 R076 AIX-3 10 Additive for High Specificity Human genomic DNA APOE 520 bp 10 Additive for High Specificity PCR 98 C2 min.

8 98 C10 C15 sec. 30 cycles68 C30 sec. 1 : Human genomic DNA 100 pg 2 : 1 ng 3 : 10 ng 4 : 100 ng 5 : 500 ng /50 l M : DL 2,000 DNA Marker 3427A 10 Additive for High Specificity 10 Additive for High Specificity M 1 2 3 4 5 M 1 2 3 4 5 M DNA 10 Additive for High Specificity 8 R076 AIX-4 10 Additive for High Specificity PCR PCR PCR Human genomic DNA 100 ng Human DCLRE1A 1 kb 10 Additive for High

9 Specificity MightyAmp PCR 98 C2 min. 98 C10 C15 cycles68 C1 min. 1 : 0 ng/ l 2 : 1 ng/ l 3 : 5 ng/ l 4 : 10 ng/ l 5 : 50 ng/ l 6 : 100 ng/ l 7 : 250 ng/ l M : DL 2,000 DNA Marker 3427A 10 Additive for High Specificity M 1 2 3 4 5 6 7 M M 1 2 3 4 5 6 7 M10 Additive for High Specificity M 1 2 3 4 5 6 7 M Taq 9 R076 AIX-5 PCR 1 Mouse Blood/FTA Card PCR mm Mouse Blood/FTA Card 25 l Mouse Hbb-b1 542 bp 3 step 30 cycles 5 l MightyAmp DNA Polymerase Mouse Blood/FTA Card

10 PCR MightyAmp PCR 98 C2 min. 98 C10 C15 cycles68 C30 sec. 1 : R071A 2 : MightyAmp Additive for High Specificity 3 : MightyAmp Additive for High Specificity 4 : B M : 100 bp DNA Ladder 3407A 2 EDTA PCR EDTA 5 l 25 l DCLRE1A 1 kb 3 step 30 cycles 5 l MightyAmp DNA Polymerase EDTA PCR PCR 98 C2 min.