ヒドロキシカルボン酸の高感度HPLC分析

HPLC . S060210B. YMC 2- (2-NPH) . 2-NPH .. HPLC UV .. A : 2-NPH .. B : [1- -3-(3- . ) ] . C : . D : . E : . 2-NPH . NO2 NO2 O. YMC-Pack FA 250 X R-COOH + NHNH2 NHNHCR. Carboxylic acid 2-Nitrophenylhydrazine 2-Nitrophenylhydrazide . 150 pmol . 1. Glycolic acid E , 2. Lactic acid 3. 3-Hydroxybutyric acid A, B 60 20 4. 2-Hydroxyisobutyric acid 4 5. 2-Hydroxybutyric acid 2 6. 2-Hydroxy-2-methylbutyric acid ( ). NO2 O 5. 7. 2-Hydroxyisovaleric acid NHNHCR 8. 2-Hydroxyisocaproic acid 6. 7.. C 60 15 3. 8. D. 1.. 0 10 20 30 min J020227A. Column : YMC-Pack FA (250 X ). Eluent : acetonitrile / methanol / water (5/40/55).. pH adjusted by TFA.. Flow rate : mL/min Temperature : 35 . Detection : UV at 400 nm, AUFS. HPLC . YMC Co., Ltd.

Column : YMC-Pack FA (250 X 6.0 mmI.D.) Eluent : acetonitrile / methanol / water (5/40/55) pH 4.5 adjusted by 0.1% TFA Flow rate : 1.2 mL/min Temperature : 35℃

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Transcription of ヒドロキシカルボン酸の高感度HPLC分析

1 HPLC . S060210B. YMC 2- (2-NPH) . 2-NPH .. HPLC UV .. A : 2-NPH .. B : [1- -3-(3- . ) ] . C : . D : . E : . 2-NPH . NO2 NO2 O. YMC-Pack FA 250 X R-COOH + NHNH2 NHNHCR. Carboxylic acid 2-Nitrophenylhydrazine 2-Nitrophenylhydrazide . 150 pmol . 1. Glycolic acid E , 2. Lactic acid 3. 3-Hydroxybutyric acid A, B 60 20 4. 2-Hydroxyisobutyric acid 4 5. 2-Hydroxybutyric acid 2 6. 2-Hydroxy-2-methylbutyric acid ( ). NO2 O 5. 7. 2-Hydroxyisovaleric acid NHNHCR 8. 2-Hydroxyisocaproic acid 6. 7.. C 60 15 3. 8. D. 1.. 0 10 20 30 min J020227A. Column : YMC-Pack FA (250 X ). Eluent : acetonitrile / methanol / water (5/40/55).. pH adjusted by TFA.. Flow rate : mL/min Temperature : 35 . Detection : UV at 400 nm, AUFS. HPLC . YMC Co., Ltd.