Transcription of GEL/PCR 産物の精製キット GEL/PCR Clean-Up Kit
1 GEL/PCR GEL/PCR Clean-Up Kit 3 3 3 4 6 * 2 300mg 100 l PCR 70-85% 90-95% PCR 20 40 l FAGCK 001 (100 preps) FAGCK 001-1 (300 preps) FADF Buffer 80 ml 240 ml Wash Buffer 25 ml (96-100%) 100ml 50 ml (96-100%) 200ml Elution Buffer 6 ml 30 ml FADF Column 100 pcs 300 pcs 2ml Collection Tube 100 pcs 300 pcs 14,000rpm 10,000 g 3 55 C 1.
2 DNA 2. (300mg ) ( 300mg 3. 500 l FADF Buffer 2% 1,000 l FADF Buffer 4. 10 -15 55 C 2-3 5. FADF Column Collection Tube 6. 800 l FADF Column 30 800 l 7. 750 l Wash Buffer ( ) 30 8. 3 9. 10.)
3 40 l Elution Buffer Elution Buffer 2 40 l 11. 2 DNA 4 4 12. DNA 4 C 20 C PCR 1. 100 l PCR 5 FADF Buffer 100 l 100 l 2. FADF Column Collection Tube 3. FADF 30 4. 750 l Wash Buffer( ) FADF Column 30 5. 3 6. 7.
4 40 l Elution Buffer Elution Buffer 2 Elution Buffer 2 40 l 8. 2 DNA 9. DNA 4 C 20 C 5 4 300mg GEL 1 300mg PCR 100 100 l pH Elution Solution DNA 5Kb Elution Solution 60 C DNA GEL: DNA DNA 95 C 2 DNA Wash Buffer 2 Wash Buffer 3 6 7 101-0032 2-13-11 TEL: 03(3864)7701 FAX: 03(3864)7752 E-mail.
