Search results with tag "Gel extraction"
PCR clean-up Gel extraction - Macherey-Nagel AG
www.mn-net.comMACHEREY-NAGEL – 02/2017, Rev. 04 3 PCR clean-up, gel extraction Table of contents 1 Components 4 1.1 Kit contents 4 1.2 Reagents, consumables, and equipment to be supplied by user 5
QIAquick Gel Extraction Kit Protocol using a microcentrifuge
lchenlab.sitehost.iu.eduQIAquick Gel Extraction Kit Protocol using a microcentrifuge This protocol is designed to extract and purify DNA of 70 bp to 10 kb from standard or low-melt agarose gels in TAE or TBE buffer. Up to 400 mg agarose can be processed per spin column. This kit can also be used for DNA cleanup from enzymatic reactions (see page 8).
In-Fusion® Snap Assembly User Manual
www.takarabio.com1. If nonspecific background bands are observed on an agarose gel, isolate your target fragment by gel extraction, then spin-column purify. 2. Spin-column purify your PCR product (e.g., insert) by using a silica-based purification system, following the manufacturer’s protocol. We recommend the NucleoSpin Gel and PCR Clean-Up kit,