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In-Fusion® Snap Assembly User Manual

In-Fusion® Snap Assembly User Manual

www.takarabio.com

1. If nonspecific background bands are observed on an agarose gel, isolate your target fragment by gel extraction, then spin-column purify. 2. Spin-column purify your PCR product (e.g., insert) by using a silica-based purification system, following the manufacturer’s protocol. We recommend the NucleoSpin Gel and PCR Clean-Up kit,

  Extraction, Clean, Gel extraction, Pcr clean

PCR clean-up Gel extraction - Takara Bio

PCR clean-up Gel extraction - Takara Bio

www.takarabio.com

PCR clean-up, gel extraction 2.3 Removal of small DNA fragments and primer-dimers NucleoSpin® Gel and PCR Clean-up is designed to remove even traces of unused primer while purifying PCR products down to 50 bp at the same time. However, in some cases it is necessary to exclude these small fragments. For example, primer dimers,

  Clean, Pcr clean

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