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Biological and molecular characterization of classical ...
Aim: The aim of this study was biological and molecular characterization of classical swine fever (CSF) challenge virus from India. ... (Ta) for 1 min, 72°C for 1 min, and a final extension of 72°C for 10 min. Annealing temperature (Ta) was standardized at 50°C, 55°C and 56°C for PCR of 5′ NTR, E2 and NS5B regions respectively (Table-1).
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