Next Generation Sequencing of Classical Swine Fever Virus

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2 Downloaded from on: Nov 28, 2021 Next Generation Sequencing of Classical Swine Fever VirusRasmussen, Thomas BruunPublished in:Workshop of the African and Classical Swine Fever National Reference Laboratories (ASF and CSF)Publication date:2015 Document VersionPublisher's PDF, also known as Version of recordLink back to DTU OrbitCitation (APA):Rasmussen, T. B. (2015). Next Generation Sequencing of Classical Swine Fever Virus . In Workshop of theAfrican and Classical Swine Fever National Reference Laboratories (ASF and CSF): Abstract book (pp.)

3 14-14).Page1-24 Workshop of the African and Classical SwineFever National Reference Laboratories (ASF andCSF) June9-102015 National Institute for Agricultural and Food Research andTechnology, (INIA).Ctra de la Coru a km 7,40028040 Madrid, Spainorganised by:EU Reference Laboratory for ASFC entro de Investigaci n en Sanidad Animal (INIA-CISA)Valdeolmos, SpainandEU Reference Laboratory for CSFU niversity of VeterinaryMedicine HannoverInstitute for VirologyHannover, GermanyIn cooperation withDG : Annex 1:Results of Interlaboratorycomparison test for CSF by National ReferenceLaboratories (NRLs) of EU member states (CSF ILCT 2014).

4 Annex 2:Results obtained in the XII Interlaboratory comparison testfor ASF2015 byNational Reference Laboratories (NRLs) of EU member states (ASF ILCT 2015). PROGRAMCSF , of and welcomeV ctor Briones (Director of CISA-INIA).Francesco Berlingieri (SANCO, EC).Marisa Arias (EURL/ASF)Paul Becher(EURL/ CSF)CSF Session I:Report of the EURL activitiesChairman: Marisa Arias (EURL for ASF) for CSF: Results of the Interlaboratory Comparison Test Austermann-Busch (CSF EURL, DE). activities during2014-2015.

5 Paul Becher & Alexander Postel (CSFEURL, DE) data base on ASF/CSF in wild boar. Christoph Staubach (DE)CSF Session II: CSF Country ReportsChairman:Francesco Berlingieri(SANCO, EC). situation in Bulgaria, Ivanova (BG). Swine Fever in Croatia-the current situation. Dragan Brnic(HR). monitoring program in Romania. Daniel Donescu (RO). situation in Latvia. Ieva Rodze (LV).CSF Session III: CSF Diagnosis and ControlChairman: Andrzej Swine Fever Virus marker vaccine strain CP7_E2alf: sheddingand dissemination studies in Petrov (DE).

6 Of a newly developed Classical Swine Fever Erns ELISAD enise Meyer (EURL for CSF, DE). of CSFV by reagents commonly used in research anddiagnosis and the effect of inactivation treatments on ELISA detectionof antigenand antibody. Helen Crooke(UK). Generation Sequencing of Classical Swine Fever Rasmussen (DK). of early infection with three attenuated strains ofclassical Swine fevervirus. Katarzyna Podgorska (PL). and long term use of a CSF antibody ELISA. Willie Loeffen(NL).

7 Discussion; conclusions and to Madrid.(Moncloa)ASF , of and welcomeV ctor Briones (Director of CISA-INIA).Francesco Berlingieri (SANCO, EC).Marisa Arias (EURL/ASF)ASF Session I: ASF Country ReportsChairman:Francesco Berlingieri(SANCO, EC). in Sardinia: a new deal in the management of eradication Feliziani (IT). current situation in Lithuania. Mindaugas Morkunas (LT). situation in Latvia. Ieva Rodze (LV). current situation in Poland. Andrzej Kowalczyk (PL) current situation in Nurmoja (EE) Session II:Report of the EURL activitiesChairman: Sophia ASFdiagnosis in affected countries, the EURL perspective.

8 CarminaGallardo (ASF EURL, ES). : Results of the Interlaboratory Comparison Test Pelayo (ASF EURL, ES). activities during 2014-2015. Marisa Arias (ASF EURL, ES). Session III: : Marie-Fr d rique Le validation of commercial and published real-time PCRmethods for detection of ASF. Adolf Steinrig,(AT). of African Swine Fever antibodies by Ingenasa ELISA in wildboar population in non-infected area, 2014 in Slovenia. Ivan Toplak(SI). table: Laboratory representatives from affected EU countriesand EURL Laboratory coordinator to share experience with questionsfrom Discussion, Conclusions and to Madrid.

9 (Moncloa) Reference LaboratoriesClassical Swine Fever and African Swine FeverWild Boar Surveillance DatabaseChristoph Staubacha, Mathias Merbotha, Stefan Kowalczyka, Thorsten Schrappsa,Sophia Austermann-Buschb, Franz-Josef Conrathsa, Marisa Arias Neirac, Paul BecherbaFriedrich-Loeffler-Institut, Institute of Epidemiology, Wusterhausen, GermanybEURL CSF, Institute of Virology, University of Veterinary Medicine Hannover, GermanycEURL ASF, Center for Animal Health Research, NationalInstitute for Agricultural and Food Research and Technology, SpainThe occurrence of CSF cases in wild boar within the EU and the successful implementation of thedatabase in recent years have clearly demonstrated the need for continuation and extensionof the period , the currently 13 participating member states entered742,363 data records into the CSF database.

10 A total of 164 defined restriction zones and vaccinationareas are recorded in the region/zone database. Furthermore, additional data for German scientificwork has been entered into the database for the period (a total of 41,352data records).Recently Austria and Latvia joined the database and are presently in a testing phase. Further EUMember States ( Estonia, Lithuania and Poland) are interested to join the database and Serbia, asaccession candidate, has also indicated its intention in maps and display of ecological information plays an important role during the evaluation processof the CSF surveillance data.