Acetone Precipitation Of Protein
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Quick-RNA™ Miniprep Kit - Zymo Research
files.zymoresearch.comMar 17, 2021 · Protein Purification: Acetone Precipitation of Proteins ü After the RNA binding to the column (page 6, step 2), the protein content in the flow-through can be purified: 1. Add 4 volumes of cold acetone (-20°C) to the flow-through (4:1) and mix. 2. Incubate the samples for 30 minutes on ice. 3. Centrifuge at max speed for 10 minutes.
Protein Sample Preparation - Bio-Rad
www.bio-rad.comavoid repeated thawing and freezing of protein samples Highly viscous samples likely have a very high DNA or carbohydrate content. Fragment DNA with ultrasonic waves during protein solubilization or by adding endonucleases like Benzonase. Use protein precipitation with TCA/acetone (for example, with the ReadyPrep 2-D cleanup kit) to
A Rapid and Sensitive Method for the Quantitation of ...
hoffman.cm.utexas.eduProtein assay (standard method). Protein solution containing 10 to 100 ,ug protein in a volume up to 0.1 ml was pipetted into 12 x 100 mm test tubes. The volume in the test tube was adjusted to 0.1 ml with appropriate buffer. Five milliliters of protein reagent was added to the