Search results with tag "Protein precipitation"
Acetone precipitation of proteins - Thermo Fisher Scientific
tools.thermofisher.com• Precipitation has an advantage over dialysis or desalting methods in that it enables concentration of the protein sample as well as purification from undesirable substances. • One disadvantage of protein precipitation is that proteins might denature, making the pellet difficult to re-solubilize.
Buffer Formulations - Bio-Rad Laboratories
www.bio-rad.comProtein Precipitation Solution (100 ml) 20% Trichloroacetic acid (TCA), 0.2% DTT in ice-cold acetone (–20°C) TCA 20.00 g DTT 0.20 g Acetone 80 ml Dissolve Acetone to 100 ml Store at –20°C Wash Solution (100 ml) 0.2% DTT in ice-cold acetone (–20°C) DTT 0.20 g Acetone 80 ml Dissolve Acetone to 100 ml Store at –20°C
Protein Precipitation for Biological Fluid Samples Using ...
www.agilent.comprotein precipitation. With this modified addition order, protein precipitation can be achieved by allowing the sample to sit for five minutes after crashing solvent addition; therefore, pipette mixing is not necessary, and can be skipped. The better mixing homogeneity achieved using this sample first, crashing solvent second method also improves
Protein Sample Preparation - Bio-Rad
www.bio-rad.comavoid repeated thawing and freezing of protein samples Highly viscous samples likely have a very high DNA or carbohydrate content. Fragment DNA with ultrasonic waves during protein solubilization or by adding endonucleases like Benzonase. Use protein precipitation with TCA/acetone (for example, with the ReadyPrep 2-D cleanup kit) to
Protein precipitation - University of Colorado Denver
www.ucdenver.eduProtein precipitation . Acetone Precipitation . Most universal . Materials: • Sample of interest • Cold (-20°C) acetone, a volume four times that of the pro tein samples to be precipitated