Autoclaving
Found 9 free book(s)Luria Broth (LB) and Luria Agar (LA) Media and Their Uses ...
asm.orgprior to autoclaving. To maximize growth, especially when a carbon source such as glucose is added, phosphate buffer or Tris-HCl buffer may be added to maintain the pH. If the medium is to be used for bacteriophage growth, a sterile stock solution of CaCl 2 is often added to a final concentration of 2.5 x10-3 M after autoclaving. Top agar (0.75 %
GENERAL AUTOCLAVE SAFETY GUIDELINES
www.ehs.washington.eduIf you are autoclaving biohazardous waste, follow the Autoclaving Biohazardous Waste Guidelines. PPE Always use personal protective equipment (PPE) when using an autoclave. Wear a lab coat, heat-resistant gloves, and safety glasses. Be sure arms are covered by a lab coat and longer heat-resistant gloves to prevent burns from
Laboratory Biological Waste Disposal Guidance
www.sgul.ac.ukAutoclaving All Biohazard group 3 organisms and Genetically Modified Organisms (GMO’s) at class 3 must be destroyed by autoclaving at 134oC. It is also preferable that Biohazard group 2 and GMO class 2 organisms are treated at 134oC however a validated chemical destruction method can be used.
AUTOCLAvED AERATED CONCRETE MASONRy UNITS
imiweb.orgOct 01, 2015 · AAC autoclaving yields a product that has a very low linear shrinkage of 0.020%. It is recommended that a breathable coating be applied on AAC wall surfaces to facilitate moisture dissipation. It is not recommended that non-breathable coatings be applied to both sides of …
LABORATORY BIOSAFETY LEVEL 2 CRITERIA
sc.eduprocessing area for decontamination, preferably by autoclaving. d. Broken glassware is not handled directly. Instead, it is removed using a brush and dustpan, tongs, or forceps. 13. Perform all procedures to minimize the creation of splashes and/or aerosols. 14.
Pulse Oximeter instructiOn manual
images-na.ssl-images-amazon.comMay 20, 2011 · Inaccurate measurements may be caused by autoclaving, ethylene oxide sterilizing, or immersing the sensors in liquid may cause inaccurate readings. Significant levels of dysfunctional hemoglobin (such as carbonyl-hemoglobin or methemoglobin) may cause inaccurate readings.
ANNEX Preparation of Media and Reagents
www.cdc.govbefore autoclaving. 2. Autoclave at 121°C for 20 minutes. 3. Cool to 60ºC in a water bath. 4. Add 5% sterile, defibrinated sheep blood (5 ml sheep blood can be added to 100 ml of agar). 2 • If a different volume of basal medium is prepared, the amount of blood added must be
Standard Safety Practices in the Microbiology Laboratory
www.who.intAutoclaving An autoclave must be available for the BSL-2/3 laboratory and must be operated only by personnel who have been properly trained in its use. To verify that each autoclave is working properly, spore strips or other biological indicators designed to test for efficiency of sterilization should be included in autoclave loads on a
Waste Disposal Procedures - Cornell University
ehs.weill.cornell.eduDATE REVIEWED: August 27, 2020 DATE UPDATED: August 25, 2020 CLASSIFICATION & LOCATION: Hazardous Waste T: \Documentation EHS-Manual 5.2WasteDisposalProcedures.docx