Catalase Test
Found 7 free book(s)
Microbiology 20 Biochemical Unknown – Fall 2008
www.lamission.edu• *oxidase test (cytochrome C oxidase) • *catalase test * Perform biochemical tests of unknown as part of Exercises 13 to 17. Adequately recording the procedures and results of each test (25 pts): • I (or anyone else) should be able to repeat your work using only your notebook
GCSE F BIOLOGY - AQA
filestore.aqa.org.ukA student did an investigation on catalase activity. This is the method used. 1. Put 1 cm3 hydrogen peroxide solution in a test tube. 2. 3Add 1 cm of catalase solution. • Bubbles of oxygen are produced. • Bubbles cause foam to rise up the tube. 3. Measure the maximum height of the foam. Figure 4 shows the experiment. Figure 4
Aerobic Gram Negative Rods Flowchart
www.cdc.govOxidase Test — used for presumptive ID only — Full Identification necessary Yes Brucella spp., Francisella spp., Eikenella spp., Pasteurella spp., Capnocytophaga Rule in/RuIe out procedure for BT agents: Francisella spp: Oxidase negative, Catalase negative or weakly positive Brucella spp: Oxidase positive, Urease positive, Catalase positive ...
Harmless Diphtheroids or Dangerous Pathogen?
www.hardydiagnostics.comhemolytic colonies that were catalase positive, Gram positive pleomorphic rods resembling diphtheroids in their morphology. A definitive rapid commercial kit identification was performed and an ID of 99% was reached after 4 hours. Hint: the test results included a positive urea. Colonies on Blood Agar at 48 hours. Gram stain . Answer and Discussion
Catalase Test Protocol - American Society for Microbiology
asm.orgThe catalase test is also valuable in differentiating aerobic and obligate anaerobic bacteria, as anaerobes are generally known to lack the enzyme (8, 9). In this context, the catalase test is valuable in differentiating aerotolerant strains of Clostridium, which are catalase negative, fromBacillus, which are catalase positive (8). Theory
CATALASE LAB - Chandler Unified School District
www.cusd80.coma. Obtain 1 vial of catalase at 100 units/mL. b. oSet up an ice bath (0C), a room temp water bath, a 37 oC bath and a boiling water bath c. Place 5 ml of catalase at 100 units/ml in each of 4 test tubes. Place 1 test tube in each of the water baths. d. Place 40 mL 1% H 2O 2 in each of 4 vials. Measure and record the depth of the H 2O 2. Place 1 ...
enzyme final lab
www.sas.upenn.edu10. Repeat procedure steps 6-9 with another of the test tubes marked “7”. 11. As a control, run the final test with the pH 7 buffer but do NOT add any enzyme. 12. Repeat procedure steps 6-11, replacing the 7 pH buffer with the pH 6 buffer, then the pH 8 buffer. 13. Be sure to print all test results. 14. Clean up all materials before leaving ...