For Nucleic Acid
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Nucleic Acid - Thermo Fisher Scientific
tools.thermofisher.comFor nucleic acid quantification, a modification of the Beer-Lambert equation is used to calculate sample concentrations. Nucleic acid sample concentrations are based on the absorbance at 260 nm, the selected analysis constant and a baseline correction. The generally accepted extinction coefficients or constants for nucleic acids
Spectrophotometric Nucleic Acid Quantification
www.biotek.comLight absorbed by the nucleic acid in the sample correlates to the concentration of nucleic acid present. Both DNA and RNA absorb light at 260 nm, therefore this is a measurement of total nucleic acid. Nucleic acid samples are also typically measured at 280 nm, which is the absorbance peak for protein. The ratio
Assessment of Nucleic Acid Purity - Yale School of Medicine
medicine.yale.edunucleic acid are often higher than the respective 260/280 values. Expected 260/230 values are commonly in the range of 2.0-2.2. Residual chemical contamination from nucleic acids extraction procedures may result an overestimation of the nucleic acid concentration and/or negatively influence downstream analysis.
Interpretation of Nucleic Acid 260/280 Ratios
tools.thermofisher.comnucleic acid absorbance at 260 nm is generally on a plateau, the absorbance curve at 280 nm is quite steeply sloped. A slight shift in wavelength accuracy will have a large effect on 260/280 ratios. It is possible to see as much as a 0.4 difference in the 260/280 ratio when measuring the same nucleic acid sample on two
Gastrointestinal Pathogen Nucleic Acid Detection Panel ...
www.uhcprovider.comGastrointestinal Pathogen Nucleic Acid Detection Panel Testing for Infectious Diarrhea Page 2 of 10 UnitedHealthcare Commercial Medical Policy Effective 03/01/2021 Proprietary Information of UnitedHealthcare.
DAPI Nucleic Acid Stain - Thermo Fisher Scientific
assets.thermofisher.comDAPI Nucleic Acid Stain | 4 2.3 Tap the tube to resuspend the pellet in the residual liquid and add 1 mL of PBS at room temperature. 2.4 Transfer the full volume of resuspended cells to 4 mL of absolute ethanol at –20°C by pipetting the cell suspension slowly into the ethanol while vortexing at top speed. Leave the cells in ethanol at –20°C for 5–15 minutes.
Nucleic Acid Pathogen Testing - Cigna
static.cigna.comA nucleic acid test analyzes tiny amounts of DNA or RNA in a sample of blood, tissue or body fluid. Because the amount of genetic material is very small the test may include a step where the DNA or RNA of the microorganism is amplified, or increased. This type of nucleic acid pathogen test is known as a nucleic acid amplification test or NAAT.