High Fidelity Dna Polymerase

PrimeSTAR® Max DNA Polymerase - Takara Bio

PrimeSTAR Max DNA Polymerase is a unique high-performance DNA polymerase that possesses the fastest extension speed available, along with the extremely high accuracy, high sensitivity, high specificity, and high fidelity of PrimeSTAR HS …

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Pfu DNA Polymerase - Agilent

Pfu DNA polymerase, a proofreading DNA polymerase isolated from Pyrococcus furiosus, is an ideal choice for a variety of techniques requiring high-fidelity DNA synthesis by the polymerase chain reaction (PCR).1–3 These applications include cloning, gene expression, and site-directed mutagenesis. Successful PCR using Pfu DNA polymerase is readily

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Thermo Scientific Phusion High–Fidelity DNA Polymerase ...

High–Fidelity DNA Polymerase is the last component added to the PCR mixture, since the enzyme exhibits 3´→5´ exonuclease activity that can degrade primers in the absence of dNTPs. Carefully mix and centrifuge all tubes before opening to …

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KOD OneTM PCR Master Mix - Toyobo

- High Fidelity . KOD One. TM. series exhibits approximately 80-fold higher fidelity than Taq DNA polymerase. The can be used for various purposes where this would be anse mixes advantage (e.g., in the preparation of long target amplicons for sequencing). - High Efficiency .

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KAPA HiFi HotStart ReadyMix PCR Kit KK2600 KK2601 KK2602

KAPA HiFi HotStart DNA Polymerase is a B-family DNA polymerase, engineered to have increased affinity for DNA, without the need for accessory proteins or DNA binding domains. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared with wild-type B-family DNA polymerases.

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KAPA HiFi PCR Kit Kapa/ Kit Codes and Components

KAPA HiFi DNA Polymerase is a B-family DNA polymerase, engineered to have increased affinity for DNA, without the need for accessory proteins or DNA binding domains. The intrinsic high processivity of the enzyme results in significant improvement in yield, speed and sensitivity when compared to wild-type B-family DNA polymerases.

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Types of mutations - Columbia University

• XP-G incises DNA 3' to the damaged site • XP-V protein is a low-fidelity class Y DNA polymerase, that can replicate UV-induced pyrimidine dimers in vivo with the insertion of the correct bases in the daughter strand • CSA likely participates in a CSB/RNA polII complex stalled at damaged sites in transcriptionally active DNA that helps

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Polymerase Chain Reaction (PCR) - University of Toledo

Polymerase Chain Reaction, 12/2004 5 MgCl 2 The concentration of MgCl 2 influences the stringency of the interaction between the primers and the template DNA. The range of MgCl 2 usually tested is from 0.5 - 4 mM in 0.5 mM increments, while …

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KOD FX Neo - Toyobo

a. Purified DNA or cDNA Appropriate template amounts for 50 μL reaction are summarized in the following table. Approved range Recommended . Genomic DNA Eukaryotic DNA 5–200 ng 50 ng Prokaryotic DNA ng 10 ng0.1–100 . Plasmid DNA 10 pgng 1 –50 ng