Illumina Adapter Sequences
Found 9 free book(s)
16S Sample Preparation Guide - Illumina, Inc.
support.illumina.comsequences,seeAmpliconPrimers,onpage3. Consumables NOTE ... MiSeqReagentKitv3(600cycle) Illumina,catalog# MS‐102‐3003 NexteraXTIndexKit Illumina,catalog# FC‐131‐1001 or Illumina,catalog#FC‐131‐1002 PhiXControlKitv3 Illumina,catalog#FC‐110‐3001 ... AnyIndex2(i5)adapter
Trimmomatic Manual: V0 - USADEL LAB
www.usadellab.orgThis step is used to find and remove Illumina adapters. Identifying adapter or other contaminant sequences within a dataset is inherently a trade off between sensitivity (ensuring all contaminant sequences are removed) and specificity (leaving all non-contaminant sequence data intact). This problem is even more acute when only a small
KAPA Library Quantification Kit Platforms
rochesequencingstore.comIllumina ® Platforms KR0405 – v9.17 ... compatibility, compare the adapter sequences used during library preparation with the library quantification primer sequences in Product Description. Ensure that the correct KAPA SYBR FAST qPCR Master …
Illumina Adapter Sequences - University of California, Davis
dnatech.genomecenter.ucdavis.eduIllumina Adapter Sequences Document # 1000000002694 v00 1 October 2015 Illumina Adapter Sequences. This document provides the nucleotide sequences that comprise Illumina oligonucleotides used in Illumina sequencing technologies. These sequences are provided for the sole purpose of understanding and publishing the results of your sequencing ...
Illumina Sequencing Technology
www.illumina.comIllumina data collection software enables users to align sequences to a reference in resequencing applications (Figure 13). Developed ... Adapter DNA fragment Dense lawn of primers Adapter ... Illumina sequencing technology leverages clonal array formation and proprietary reversible terminator technology for rapid and accurate large-scale ...
Understanding Illumina TruSeq Adapters - Tufts University
tucf-genomics.tufts.eduThe TruSeq Universal adapter and the Indexed adapter have a very short region where they are complimentary. Examining the 5’ 3’ of the TruSeq Universal Adapter and the 3’‐5’ Sequence of the Indexed adapter we see: The only complementary sequence is the last 12 nucleotides. In order to take these individual
Illumina Adapter Sequences (1000000002694)
dnatech.genomecenter.ucdavis.eduAdapter i7Basesfor SampleSheet i5BasesforSampleSheet NovaSeq,MiSeq, HiSeq 2000/2500 i5BasesforSampleSheet iSeq,MiniSeq,NextSeq, HiSeq 3000/4000 UDP0001 CGCTCAGTTC GAACTGAGCG TCGTGGAGCG CGCTCCACGA UDP0002 TATCTGACCT AGGTCAGATA CTACAAGATA TATCTTGTAG UDP0003 ATATGAGACG CGTCTCATAT TATAGTAGCT …
Nextera XT DNA Library Prep Kit Reference ... - Illumina, Inc.
support.illumina.comTherefore,Illumina recommends that amplicons < 500 bp undergo a 1.8 x sample purification beadvolume ratio to supernatantduring Clean Up Libraries on page 1. Shorter amplicons can otherwisebe lost during the library cleanupstep. Tagmentation cannot add an adapter directly to the distal end of a fragment,so a dropin sequencing
AnintroductiontoNext-Generation Sequencing Technology
www.illumina.comFigure 2:HumanGenomeSequencingOvertheDecades—Thecapacitytosequenceall3.2billionbasesofthehumangenome(at30×coverage)hasincreased exponentiallysincethe1990s.In2005 ...