Lactose Broth
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Chapter 6: Microbial Nutrition and Growth
mymission.lamission.edu• Nutrient broth: Liquid media ... •Lactose plus pH indicator: Lactose fermenters produce pink or red colonies, nonfermenters are colorless. Microbial Growth Culture Media Enrichment Culture: Used to favor the growth of a microbe that may be found in very small numbers.
Chapter 10 - MICROBIOLOGICAL ANALYSES
www.who.intIn the second (confirmation) part, brilliant green lactose bile (BGLB) broth is used to confirm total coliforms and E. coli medium to confirm faecal coliforms. Some of the characteristics of these and other media suitable for use in most probable number analyses are described in Table 10.2. Media can be made from primary ingredients but are also
ENUMERATION OF MICROORGANISMS I. OBJECTIVES II. …
www.sas.upenn.eduLactose-bromcresol purple broth 9/group Saline tubes same as viable count Pipettes, 5 ml 5/group IV. PROCEDURES (Direct count is done by each student individually; however, the students at a table will form a group to perform the viable count and the MPN.) First Session Direct Count Using a Counting Chamber 1.
Lactose Broth - HiMedia Labs
himedialabs.comPlease refer disclaimer Overleaf. Lactose Broth M1003 Intended use Lactose Broth is used for the detection of coliform bacteria in water, foods, dairy products and clinical samples.
A. Enrichment in Alkaline Peptone Water
www.cdc.govbroth, since vibrios preferentially grow in this area. Do not shake or mix the tube before subculturing. If the broth cannot be subcultured after 6 to 8 hours of incubation, subculture at 18 hours to a fresh tube of APW. This second tube should be subcultured to a solid medium after 6 to 8 hours incubation.
MacConkey Broth - HiMedia Labs
himedialabs.comPlease refer disclaimer Overleaf. MacConkey Broth MH083 Recommended for the selective enrichment of E.coli from pharmaceutical products in accordance with the microbial
Low-Fiber/Low-Residue Diet - University of Michigan
medicine.umich.edu• Limit milk and milk products to 2 cups per day. Use lactose-free products if you are lactose intolerant. • Limit foods high in fat. Breads, Cereals, Rice and Pasta Foods Recommended Foods to Avoid 6-11 servings each day . Serving Size = 1 slice bread, 1 cup cooked cereal, rice or pasta
3.8 Bacterial Identification Techniques - FWS
www.fws.govi. Inoculate tryptone broth (Section 2, 3.7.B.6 “Tryptone Broth”) with a light inoculum from an 18 to 24 hour pure culture. ii. Incubate 24 to 48 hours at 20oC iii. At the end of 24 hours incubation do the following: 1. Aseptically remove 2 mL of media and place in an empty sterile test tube. Save extra 2. 3. 1.
RAPID’Salmonella Agar Short Protocol - Bio-Rad
www.bio-rad.comRAPID’Salmonella Agar Short Protocol RAPID’Chromogenic Methods • Food Testing Quick response Detection of Salmonella spp. in less than 42 hours Easy protocol Only 1 single broth and 1 single solid agar plate Easy confi rmation Salmonella Latex Meets the regulations Suitable as second medium in the ISO 6579 standard
Gastroparesis Nutrition Therapy
www.med.umich.eduSoups: Broth-based, bouillon, strained creamed soups (with milk or water) Beverages: Hot cocoa (made with water or milk), Gatorade or Powerade, soft drinks, coffee/coffee drinks, tea/ chai, 100% fruit and vegetable juices, dairy or
Indole Test Protocol - American Society for Microbiology
asm.orgferment lactose, producing acid and gas) (5). The indole test is still used as a classic test to distinguish indole-positive E. coli from indole-negativeEnterobacter and Klebsiella. (8) Purpose The indole test screens for the ability of an organism to degrade the amino acid tryptophan and produce indole. It is used as part of the
SELECTIVE AND DIFFERENTIAL MEDIA
fire.biol.wwu.eduOvernight broth cultures of Staphylococcus aureus, Staphylococcus epidermidis, and Enterococcus faecalis (on plate). 4. Sterile swabs and sterile toothpicks 5. Rabbit plasma 6. 1.0 and 0.1 ml pipets 7. 3% Hydrogen peroxide solution Procedure: (work in pairs) 1. Swab an area of the face (e.g., cheek or forehead) and streak a mannitol salt agar ...